3D cell culture is an artificially-created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions

3D cell culture is an artificially-created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies. Approximately 300 spheroids are usually cultured per bioreactor.

Cell-Mate is a biomimetic 3D cell culture matrix designed to support and enhance your 3D cell culture assays. Materials are carefully chosen and processed to create an ideal microenvironment for your cells. Cell-Mate™ is also injectable, offering seamless translation from your incubator to your animal model.

Cell-Mate3D™ is an injectable, tissue-like matrix that offers researchers a biologically relevant microenvironment for in vitro and in vivo biomedical research. It is chemically defined: composed of naturally occurring biopolymers Hyaluronic Acid (HA) and Chitosan (CT).

• HA is a linear polysaccharide found in the extracellular matrix of connective, epithelial, and neural tissues. It is involved in cell proliferation, migration, embryonic development, and wound healing. HA also plays a role in cancer biology.
• CT, derived from Chitin, is a positively charged polysaccharide that is found in the exoskeleton of crustaceans.

When Cell-Mate3D™ dry blend is combined with cells in hydration fluid, Cell-Mate3D™ matrix forms instantly through electrostatic interactions between HACOO^–groups and CTNH3⁺ groups. This interaction results in Poly-Electrolytic-Complex fibers that give structure to the construct.